Dansyl- and rhodamine-based fluorescent sensors for detecting singlet oxygen and superoxide production in plants in vivo
Reactive oxygen species (ROS) play a key role in a variety of biotic and abiotic stress conditions, therefore their direct measurement in vivo is of special importance. In this study, we describe two dansyl-based ROS sensors, the singlet oxygen specific DanePy; HO-1889NH, which is reactive to both s...
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2002
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| Sorozat: | Acta biologica Szegediensis
46 No. 3-4 |
| Kulcsszavak: | Természettudomány, Biológia |
| Online Access: | http://acta.bibl.u-szeged.hu/22539 |
| Tartalmi kivonat: | Reactive oxygen species (ROS) play a key role in a variety of biotic and abiotic stress conditions, therefore their direct measurement in vivo is of special importance. In this study, we describe two dansyl-based ROS sensors, the singlet oxygen specific DanePy; HO-1889NH, which is reactive to both singlet oxygen and superoxide radicals and a rhodaminebased singlet oxygen sensor, HO-2941. In order to characterise the potential use of these chemosensors in plant experiments in vivo, we characterise their ROS trapping ability, fluorescence emission spectra and localisation in spinach leaves. |
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| Terjedelem/Fizikai jellemzők: | 149-150 |
| ISSN: | 1588-385X |