Determination and uncertainty analysis of inorganic arsenic in rice by UHPLC-ICPMS
The present study arose from the need of to determine inorganic arsenic (iAs) at low levels in rice. Ultra-high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (UHPLC-ICPMS) using Kinetic Energy Discrimination (KED) mode to eliminate spectral interferences...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2020
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| Sorozat: | Acta biologica Szegediensis
64 No. 2 |
| Kulcsszavak: | Biológia, Kémia |
| Tárgyszavak: | |
| doi: | 10.14232/abs.2020.2.251-259 |
| Online Access: | http://acta.bibl.u-szeged.hu/73075 |
| Tartalmi kivonat: | The present study arose from the need of to determine inorganic arsenic (iAs) at low levels in rice. Ultra-high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (UHPLC-ICPMS) using Kinetic Energy Discrimination (KED) mode to eliminate spectral interferences was used for analysis of iAs. Sample preparation involves extraction of inorganic arsenic (sum of As3+ and As5+) with water by heating at 90 °C for 5 min in water bath. Separation is accomplished with a reversed-phase ion pack column using a gradient chromatographic method followed by ICPMS analysis within 5 min. The method was validated in accordance with Commission Regulation (EU) No 836/2011 and performance characteristics were verifi ed. Acceptable values were obtained for specifi city, repeatability (HorRatr < 0.6), within-lab reproducibility (HorRatR < 0.3) with recovery 80-90%, limit of quantifi cation (0.02 mg/ kg), fi tness-for-purpose and trueness (using CRM); thus, the method can be considered for offi cial control purposes. |
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| Terjedelem/Fizikai jellemzők: | 251-259 |
| ISSN: | 1588-4082 |