TLC analysis to determine the seasonal dependence of the level of 20-hydroxyecdysone in the leaves of Serratula tinctoria (L)
A quantitative method has been developed for determination of the 20-hydroxyecdysone content of Serratula tinctoria (L.), Thin-layer chromatography on silica was performed in two consecutive steps; the first was with chloroform - ethanol and the separation was completed with chloroform - methanol -...
Elmentve itt :
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Dokumentumtípus: | Cikk |
Megjelent: |
1996
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Sorozat: | JPC - JOURNAL OF PLANAR CHROMATOGRAPHY - MODERN TLC
9 No. 4 |
Tárgyszavak: | |
mtmt: | 1723678 |
Online Access: | http://publicatio.bibl.u-szeged.hu/31766 |
Tartalmi kivonat: | A quantitative method has been developed for determination of the 20-hydroxyecdysone content of Serratula tinctoria (L.), Thin-layer chromatography on silica was performed in two consecutive steps; the first was with chloroform - ethanol and the separation was completed with chloroform - methanol - benzene. Plant samples of different phenophases were collected in 1994, ecdysteroids were extracted, and, after a clean-up procedure, the 20-hydroxyecdysone content was determined by TLC - densitometry. |
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Terjedelem/Fizikai jellemzők: | 264-268 |
ISSN: | 0933-4173; 1789-0993 |